This course deals with Basic Tools in the Molecular Biology Laboratory, Structure of DNA & RNA, Gene regulation in prokaryotes, Cutting and joining DNA molecules, PCR, Blotting techniques. Isolation of plasmid DNA from bacteria - principle. Isolation of plant genomic DNA - principle. Molecular weight determination of DNA using Agarose gel Electrophoresis. Restriction Fragment Length Polymorphism, Randomly Amplified Polymorphic DNA-PCR