ARBA MINCH UNIVERSITY
COLLEGE OF NATURAL SCIENCES
DEPARTMENT OF BIOLOGY
COURSE GUIDEBOOK
Module Name: Optional III
(Plant Tissue Culture and Food Biotechnology)
Course Title: Introduction to Plant Tissue Culture and Propagation
Course Code: Biol 3131
Course Credit 2 (1 theoretical classes + 1 practical /week)
Course Credit point: 3 ECTS
Pre-requisite Plant Physiology (Biol2024)
Academic Year 2015 GC/2007 EC
Semester II
Target group III year, II semester Biology major students
Course status Elective
Instructors contact information
Instructors Name Dr. Ch. Bhaskar Rao
E-mail chinthapalli.bhaskar@amu.edu.et
Office Number
Office phone number
Office hour
Class schedule
Venue
1. Course Description:
Laboratory organization of plant tissue culture; medium and sterilization techniques; different types of cultures; micro propagation; cell suspension and secondary metabolites; in vitro production of haploids; protoplast isolation and fusion; somaclonal variation; germplasm storage and cryopreservation; micropropagation and other propagation methods
2. Course objectives
After completing the course, the students should be able to:
Master the basic principles and skills regarding techniques, practices and procedures of plant tissue culture (Micropropagation), asepsis, laboratory plan, equipment and facilities, and green house growing.
• Discuss applications of plant tissue culture.
• Practice the most common methods of plant cell and tissue culture.
• Propagate rare and endangered plant species by laboratory method.
3. Student Workload:
Students will spend 81 hours on the course (3 * 27) which will be distributed approximately to the following activities.
Activity Hour
Lecture 16
Laboratory work 21
Assignment and Presentation 13
Independent study 23
Assessment 8
Total 81
4. Teaching Learning Methods:
Lecturing
Group discussion and Presentation
Reading assignments and Reporting
Laboratory report writing
Demonstration
5. Tentative Course Schedule
Time Topics/Contents Readings
Week one 1. Introduction to Plant tissue culture George, E., M. Hall, G. Klerk (eds.), 2007. Plant propagation by tissue culture. 3 edition. vol.1
1.1. Definition
1.2. History
1.3. Applications
Week two 2. Facilities and supplies George, E., M. Hall, G. Klerk (eds.), 2007. Plant propagation by tissue culture. 3 edition. vol.1
2.1. Organization of a tissue culture laboratory
2.2. Equipment and supplies
3. Tissue culture media
3.1. Media components
3.2. Media preparation
Week three 4. Callus and cell culture George, E., M. Hall, G. Klerk (eds.), 2007. Plant propagation by tissue culture. 3 edition. vol.1
4.1. Callus induction
4.2. Callus culture
4.3. Cell suspension culture
Test One=10%
Week Four 5. Micropropagation George, E., M. Hall, G. Klerk (eds.), 2007. Plant propagation by tissue culture. 3 edition. vol.1
5.1. Applications
5.2. Micropropagation types
5.3. Micropropagation stages
Week Five 6. Organogenesis and Embryogenesis George, E., M. Hall, G. Klerk (eds.), 2007. Plant propagation by tissue culture. 3 edition. vol.1
7. Haploid Production -Anther/microspore culture
Test Two=10%
Week six 8. Embryo culture George, E., M. Hall, G. Klerk (eds.), 2007. Plant propagation by tissue culture. 3 edition. vol.1
9. Protoplast culture
10. Somaclonal variation
Assignment=10%
Week Seven 11. Plant transformation George, E., M. Hall, G. Klerk (eds.), 2007. Plant propagation by tissue culture. 3 edition. vol.1
12. Germplasm preservation
Laboratory schedule for the course Biol 3131
Time Activity Topics/Contents Readings
Week three Session one Media Preparation Laboratory manual
Week four Session two Meristem culture (could be done from many plant species Laboratory manual
Week five Session three Callus culture (Initiation and regeneration) Laboratory manual
Week six Session four Haploid culture (pollen could be used Laboratory manual
Week seven Session five Protoplast isolation Laboratory manual
6. Mode of Assessment Methods:
Tests …………………………………………………. .. 20%
Assignment…………………………………………….. 10%
Laboratory Activities and Reports.…………………… 20%
Final written exam………………….……..………… 50%
Total ………………………………………………… 100%
7. Course policies:
The instructor expects that students will have read the appropriate chapters in the reference books BEFORE the class. Students are responsible for all material covered in lecture, whether it comes directly from the text or not. Students arrive on time to class. We will conduct this class in an atmosphere of mutual respect. The instructor encourages your active articipation in class discussions as well as in laboratory sessions. You should expect that if your conduct during class discussions seriously disrupts the atmosphere of mutual respect we expect in this class, you will not be permitted to participate further and penalized for your act. If you feel that you need individual help or would like to come by to talk (about this course or anything else), please come by my office during office hours, or schedule a time that is more convenient.
8. References:
1. Agrawal, R.L., 1998.Fundamentals of plant breeding and hybrid seed production. Oxford and IBH publishing, New Delhi.
2. Bhojwani, S. and M. Razdan, 1996. Plant tissue culture: Theory and Practice. Department of Botany University of Delhi. Amsterdams, the Netherlands, revised edition. pp. 26 - 33
3. George, E., M. Hall, G. Klerk (eds.), 2007. Plant propagation by tissue culture. 3edition.vol.1
4. IAEA (International Atomic Energy Agency), 2004. Low cost options for tissue culture technology in developing countries. Proceedings of a technical meeting organized by the joint FAO/IAEA division of nuclear techniques in food and agriculture and held in Vienna, 26-30.
http://www-pub.iaea.org/MTCD/publications/PDF/te_1384_web.pdf
5. Mineo, L., 1990. Plant tissue culture techniques.
http://www.ableweb.org/volumes/vol11/9-mineo.pdf
6. PTL (PhytoTechnology Laboratories), 2008. African violet multiplication kit.
http://www. phytotechlab.com/ pdf/ African VioletKit.pdf
7. Seymour, W. Mitchell and M. Ahmad, 2003. A novel surface sterilization method for reducing microbial contamination of field grown medicinal explants intended for in vitro culture. Biotechnology Centre, U.W.I, Mona, Kingston 7, Ja. W.I.Part II
Checked by Approved by
Name:___________________ Name:____________________
Signature:_________________ Signature:_________________
- Teacher: Chinthapalli Bhaskar